Vertical Agarose gels, help

Mr. M.J. Lush mlush at hgmp.mrc.ac.uk
Tue Jun 4 09:11:44 EST 1996


In article <4mpo5o$1f92 at power.ci.uv.es>,
Inmaculada Garcia  <garciai at uv.es> wrote:
>Dear colleagues:
>
> I am trying to run a vertical agarose gel, (0.5% agarose), but I am 
>having quite a lot of problems with it. The main difficulty is to prepare 
>the gel avoiding its breakage or its slippage from the glasses. The 
>second one is to take out the comb without breaking the slots.
>
> Could anyone give me any tip to prepare it properly?

	Have you tryed getting one or both of the plates frosted?
And while were talking about cold :-) does chilling the gel when removeing
the comb help?

-- 
Michael

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
NPC rights activist           |      Nameless Abominations are people too.



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