? Counting Cells using a desktop scanner ?

[Dennis Templeton]

In article <4okn15$95n at sparcserver.lrz-muenchen.de>,
diabetes at lrz.uni-muenchen.de (Wolfgang Schechinger) wrote:

> Did anyone ever try to count cells in a dish using a desktop scanner?
> My idea was staining the cells (e.g. smooth muscle cells) with a high
> contrast stain, then taking a microphotograph and scanning in the
> picture. Cells the could be counted with an appropriate software. Any
> experience / ideas?
> 
> Please mail your answeres, too.

A plug in for NIH IMage does pretty much that. IMage is at
ftp://zippy.nimh.nih.gov/pub/nih-image/

I have used image, but not that plug-in, so you'll have to work out the
details for yourself.

Dennis

Dennis Templeton
Institute of Pathology
CWRU School of Medicine
djt2 at po.cwru.edu