Help: MORPH and Stratagene mutagenesis kits
A.S. Sohal
u9310871 at MUSS.CIS.MCMASTER.CA
Tue Jun 4 08:38:38 EST 1996
Hi! My name is Avtar and I have been experimenting with some new
site-directed mutagenesis kits. In particular, I have been working with
the MORPH single-primer site-directed mutagenesis kit and the Chameleon
site-directed mutagenesis kit from Stratagene. I was wondering if anyone
has had any success with these kits. I am having trouble isolating my
treated plasmid from the mut S cells after transformation. The cells
grow in LB-amp so the transformation to gain amp resistance must have
been successful. However, attempting to isolate the plasmid by means of
a alkaline lysis mini-prep has been unsuccessful in producing any DNA. I
hope someone can save me.
Thanx
Avtar
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