HELP HELP cDNA library

Els Van Geldre Els.VanGeldre at rug.ac.be
Wed Jun 5 09:59:47 EST 1996


Hi,

I synthetised cDNA starting from mRNA from plant sources. After addition 
of Eco RI/Not I adaptors and insertion into lgt 11 phage I only become 
blue plaques. The problem is probably the addition of the 
EcoRI/Not I adaptors or the insertion into the lgt 11 phage. However, I 
strictly followed the instructions of the kit I used (TimeSaver cDNA 
synthesis kit). Do you have any suggestions to become recombinant plaques 
?

Thanks,
Els Van Geldre




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