subcloning

Michael Witcher mwitcher at cs.mun.ca
Tue Jun 4 06:37:07 EST 1996


 I am trying to subclone a 2900 bp insert into a bluescript vector,also ~2900 bp in length, and it's not working. The main problem is that the dephosphorylation rxn. with CIAP isn't working. Does anyone have a good subcloning protocol?
w
w:wq
:wq
w




More information about the Methods mailing list