subcloning

Michael Witcher mwitcher at cs.mun.ca
Mon Jun 3 14:08:14 EST 1996


I am having great difficulty cloning a cDNA insert(~2900 bp) into a bluescript vector (~2900 bp). The problem seems to lie mainly in the dephosphorylation rxn. Does anybody have a good, detailed protocol for subcloning? Even though cloning an insert into a vector of the same size is difficult, it should work if the molecular ratio of insert:vector is high enough.
 



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