CNBr Cleavage of soluble proteins

[Michael West]

I am gearing up to do CNBr cleavage of candidates from a library of 
fusion proteins.  I am cleaving a leader sequence from the C-terminal
end and a antibody and His-tag from the N-terminal end.  The resulting
protein is ~8kd and should be soluble.

I would like to hear from anyone who has successfully performed
this type of cleavage and/or I would like to find a good references 
on the subject.

Thanks,
Michael
-- 
"...none of the tears that we cry in sorrow or rage
        Can make any difference, or turn back the page..." -DG
 ************************************************************************                                                 
Michael West				micky at berthaw.princeton.edu