Cell cycle analysis by FACS

joestudent at mit.edu joestudent at mit.edu
Thu Jun 6 14:32:57 EST 1996


Protocol I use:

Trypsinize (unless looking at cell surface antigens/markers)
Spin down
Wash with PBS 1% serum
Spin down
Resuspend in 200 microliters PBS
Fix with 2mls 95% ETOH at 4 degrees

At this point the cells can stay in ETOH at 4 for days (weeks?)

When ready for PI staining spin down and wash with 1 ml PBS/serum

Scott Estes PhD
MIT



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