Lambda DNA Problems

tjstbc at SD.CTS.COM tjstbc at SD.CTS.COM
Thu Jun 6 13:38:46 EST 1996


I am having problems in cutting lambda DNA prepared from liquid lysates
using Promega's LambdaSorb and Qiagen Lambda Kit. I have done
Phenol/chloroform extractions, as well as ethanol precipitations to help
remove high salt concentrations. I have also treated a sample with
agarase and still there is no cutting. Can anyone give me suggestions on
how to help with my situation?

Steve Nario
Tera Biotechnology Corporation





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