2D PAGE pH gradient
egreif at cc.umanitoba.ca
Thu Jun 6 12:07:07 EST 1996
Has anyone tried to measure the pH of the IEF gel?
I've done it by cutting the IEF in 0.5cm pieces, soaking each piece in
300-400uL of water overnight in the cold room and reading them the next
day. It takes a long time the pH to stabilize in order to get a reading to
see if the ampholytes are working.
egreif at ccu.umanitoba.ca
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