Colcemid or colchicine cell culture arrest conditions/method?
075bert at chiron.wits.ac.za
Fri Jun 7 13:57:15 EST 1996
could anyone out there please give me a protocol for using colchicine or
colcemid for arresting Leukaemic cell cultures in metaphase? We are
switching from TC arresting solution (no longer available as far as I know).
If you have info on concentrations and time of exposure (including overnight
methods) please let me know.
Thanks for any help in advance
075HERB at wits.ac.za
Univ Witwatersrand Med School
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