Colcemid or colchicine cell culture arrest conditions/method?

Herbert Ludewick 075bert at
Fri Jun 7 13:57:15 EST 1996

could anyone out there please give me a protocol for using colchicine or 
colcemid for arresting Leukaemic cell cultures in metaphase? We are 
switching from TC arresting solution (no longer available as far as I know).
If you have info on concentrations and time of exposure (including overnight 
methods) please let me know.
Thanks for any help in advance

075HERB at

Univ Witwatersrand Med School
South Africa

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