2D nightmare
jgluz at STUD.MED.CORNELL.EDU
jgluz at STUD.MED.CORNELL.EDU
Sat Jun 8 00:05:38 EST 1996
I am trying to run the first dimension of a 2D gel using the Biorad mini
gel system. I cannot get them to run. At first, they run at about 750V
with a current of about 10 mAmps. Eventually, the resistance gets so
high they just won't run at all (about thirty minutes). No matter what
power supply I use, the current slowly drifts down to 1 mAmp and then
the power supply goes to fault. I've gotten all kinds of explanations
from Biorad but none is satisfactory. Has anyone ever gotten this thing
to work? All advice, specific and general, is welcome.
--jgluz at stud.med.cornell.edu
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