2D nightmare

jgluz at STUD.MED.CORNELL.EDU jgluz at STUD.MED.CORNELL.EDU
Sat Jun 8 00:05:38 EST 1996


I am trying to run the first dimension of a 2D gel using the Biorad mini 
gel system.  I cannot get them to run.  At first, they run at about 750V 
with a current of about 10 mAmps.  Eventually, the resistance gets so 
high they just won't run at all (about thirty minutes).  No matter what 
power supply I use, the current slowly drifts down to 1 mAmp and then 
the power supply goes to fault.  I've gotten all kinds of explanations 
from Biorad but none is satisfactory.  Has anyone ever gotten this thing 
to work?  All advice, specific and general, is welcome.					
--jgluz at stud.med.cornell.edu  






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