Attachment methods fro chromophore onto a protein

M Thompson mthompson at asu.edu
Fri Jun 7 19:18:07 EST 1996


I am looking for methods to attach a chromophore, such as thiazole orange
and yellow, to a cysteine at residue 1 via a disulfide bridge. One idea is
the disufyl-dipyridyl activation of either the chromophore or the protein,
with subsequent covalent bond formation of the other. I was wondering if
there is an individual who may have experimented with this reaction or has
thoughts on this approach or possibly another method. 

Thanks in advance.



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