Attachment methods fro chromophore onto a protein

M Thompson mthompson at
Fri Jun 7 19:18:07 EST 1996

I am looking for methods to attach a chromophore, such as thiazole orange
and yellow, to a cysteine at residue 1 via a disulfide bridge. One idea is
the disufyl-dipyridyl activation of either the chromophore or the protein,
with subsequent covalent bond formation of the other. I was wondering if
there is an individual who may have experimented with this reaction or has
thoughts on this approach or possibly another method. 

Thanks in advance.

More information about the Methods mailing list