Using old slides for FISH
dauw at ruly46.medfac.leidenuniv.nl
Fri Jun 7 03:33:28 EST 1996
Does anybody have a method to regerated old slides to use
them succesfully in FISH?
The problem is that slides stored at RT or 4C dry out and
will not hybridize anymore, than you have lost your precious
It sometimes work to do a pre-treadment with RNase and
proteinase K (or pepsin) followed by a post fixation with
formaldehyde. But this works only in a few cases.
More information about the Methods