PEG in ligations, why?

John Elliott johne at gdpiris.chem.sunysb.edu
Sat Jun 8 01:55:56 EST 1996


PEG also helps in lining up the dna fragments by increasing viscosity and
effectively extending the random structure of the dna. Sometimes helpful
for ligation of large inserts. You are more likely to get tandom ligation
if you have the same restriction site at the ends of a short insert.

jte



More information about the Methods mailing list