DNAase step in differential display PCR

Louis Alarcon lha at med.pitt.edu
Sat Jun 8 09:46:07 EST 1996


In article <4pan98$n6 at neuro.usc.edu>, william at neuro.usc.edu (William Sun) wrote:

> After isolation of mRNA for differential display PCR, is it necessary to
> treat the mRNA with DNAase?  I would like to omit extra steps if possible
> to prevent RNAase contamination.


If you are using anchored oligo-dTs as 3' primers for DD-PCR, it is not
necessary to DNAse treat the starting RNA, since the oligo-dT will specify
amplification of poly-A mRNA only.

Lou Alarcon
Dept of Surgery
U of Pittsburgh



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