DNAase step in differential display PCR
lha at med.pitt.edu
Sat Jun 8 09:46:07 EST 1996
In article <4pan98$n6 at neuro.usc.edu>, william at neuro.usc.edu (William Sun) wrote:
> After isolation of mRNA for differential display PCR, is it necessary to
> treat the mRNA with DNAase? I would like to omit extra steps if possible
> to prevent RNAase contamination.
If you are using anchored oligo-dTs as 3' primers for DD-PCR, it is not
necessary to DNAse treat the starting RNA, since the oligo-dT will specify
amplification of poly-A mRNA only.
Dept of Surgery
U of Pittsburgh
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