cwen at aux.btny.purdue.edu
Sat Jun 8 20:36:41 EST 1996
In article <199606080501.BAA29479 at gramercy.ios.com>, jgluz at STUD.MED.CORNELL.EDU wrote:
>I am trying to run the first dimension of a 2D gel using the Biorad mini
>gel system. I cannot get them to run. At first, they run at about 750V
>with a current of about 10 mAmps. Eventually, the resistance gets so
>high they just won't run at all (about thirty minutes). No matter what
>power supply I use, the current slowly drifts down to 1 mAmp and then
>the power supply goes to fault. I've gotten all kinds of explanations
>from Biorad but none is satisfactory. Has anyone ever gotten this thing
>to work? All advice, specific and general, is welcome.
>--jgluz at stud.med.cornell.edu
Supposedly, if every condition you have used is right, you should observe a
decrease in the current conductivityafter the proteins have reached their pIs.
At this point, the proteins can not conduct the current.
Therefore you encountered a very high resistance, according to V=IR, where V
is voltage, I is current and R is resistance. You should set constant
amperage to run the IEF gel, but not constant voltage.
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