ExoIII-Why not ApaI?
Markus.Piotrowski at rz.ruhr-uni-bochum.de
Sat Jun 8 21:38:43 EST 1996
somewhere I read, that some restriction enzymes -although leaving a 4
base 3' protusion - can't be used to protect DNA in an ExoIII-digestion.
Fortunately I read this after using such an enzyme, ApaI, and I found
that it worked well in my case.
The only problem was the complete removal of some bases (from the
ApaI-site to the first base of the KpnI-site in pBs SK+) on the
Is this the reason why these enzymes shouldn't be used or why are they
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