suicidal rna extracter
gzk at ollamh.ucd.ie
Mon Jun 10 05:07:14 EST 1996
Can anyone help us with our rna extractions? We're using Ultraspec
( chomsinsky's method) to extract hepatitis C rna from serum.
When it works we get a nice product but it seems to be a bit
of a hit and miss affair . I think we may be missing those
specimens with a lower target number. We often see largish pellets
after precipitation overnight with glycogen but get no product from
the rt-pcr. Our problem may be the quality of the water we dissolve
the pellet in . Any ideas? many thanks.
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