non-radioactive PCR...any suggestions?
salger at zi.biologie.uni-muenchen.de
Mon Jun 10 13:50:34 EST 1996
> I am doing differential display (Liang & Pardee). Has anyone used a
> non-radioactive approach to amplify cDNAs after RT? Should I get
> custom-labelled primers or should I label them myself with biotion?
people down the hall developed a non-radioactive protocol for
differential display. It was published in BioTechniques.
REN Display, A Rapid and Efficient Method for Nonradioactive Differntial
Display and mRNA Isolation
LOHMANN, J.; SCHICKLE, H.; BOSCH, T. C. G.
Benchmark, BioTechniques, 18:200-202
You don't need labeled primers for this method. Rather it uses silver
staining to detect the bands. People here are using a Pharmacia
MultiPhor gel chamber and precast gels. They say these gels work much
better than the usual home made ones.
If you are interested you could contact Jan Lohmann via e-mail:
lohmann at zi.biologie.uni-muenchen.de
Hope this helps
Klaus Salger phone : +49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG MacWilliams e-mail: salger at zi.biologie.uni-muenchen.de
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