nuclear runoff assay

mahayden at med.unc.edu mahayden at med.unc.edu
Mon Jun 10 12:39:13 EST 1996


    I am attempting to perform the nuclear runoff assay on rat pulmonary 
smooth muscle cells. My problem lies in the isolation of the nuclei.  
I'm currently trying to isolate them using a sucrose gradient.  Any 
suggestions (or protocols) on nuclei isolation greatly appreciated!

 M. Hayden




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