phenol stories

john wolff wolffie at u.washington.edu
Mon Jun 10 11:39:53 EST 1996


In article <4opkis$47q at mserv1.dl.ac.uk>, PROUET
<100550.3012 at CompuServe.COM> wrote:

> Hi all !
> 
> We experienced a few years ago that if DNA was mixed with low grade phenol
> and even if you removed the phenol with several ethers or chloroforms....
> The DNA was impossible to cut ! We solved this problem with using some
> more expensive phenol.

We haven't used phenol for years.  We use a chloroform salting-out
extraction for genomic DNA.  Lots of DNA extraction kits on the market now
that don't use phenol.



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