single cell RT-PCR

Szabadkai Gyorgy SZABADKAI at PUSKIN.SOTE.HU
Mon Jun 10 11:21:33 EST 1996


Hi,

I have a question about RT protocol for single cell RT-PCR. We use a 
10 min rt - 20 min 42C - 5 min 99C protocol with 50U of MMLV RT H 
plus. Does it makes sense to make the reaction for longer time or to 
use more RT enzyme to get better reproducibility?

Thanks,


szagyo



More information about the Methods mailing list