FISH and Banding... What is a good technique (R or G??) ?
dellaire at ODYSSEE.NET
Tue Jun 11 07:21:22 EST 1996
I would like to band chromosomes of murine and human fibroblasts.
I am doing one and two color FISH and I would like to see if my signals
are in early or late replicating bands. The lab I am in works with
R banding but in peripheral blood lymphocytes. The R technique we use
involves BrdU and thymidine block and seems laborious. Is there other R
banding techniques that are less laborious, or alternately can I use a
simple G banding protocol (trypsin?) to obtain the banding for FISH.
Thanks in advance for all suggestions
McGill Dept. of Medicine
Div. of Exp. Medicine
dellaire at odyssee.net
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