IS-hybridisation: emulsion problem
Mr. M.J. Smith
mjsmith at hgmp.mrc.ac.uk
Tue Jun 11 05:56:57 EST 1996
I have a problem with some brain sections hybridised with an 35S labelled
oligo probe, coated with Amersham LM-1 autoradography emulsion.
A dense, yellow-brown non-specific stain is affecting the tissue
sections. I have used the technique in the past with no problem and my
feeling is that something is degrading over time in the emulsion. The
staining is not due to light contamination (there are no background
silver grains but a signal is appearing in appropriate areas), and is not
due to the developing process.
I have contacted Amersham who are going to investigate the batch of
emulsion. In the mean time I would be interested if anyone has had
similar problems and what the cause is. Alternatively this is a dodgy
batch of emulsion of which collegues should be aware, as lengthy
experiments may be ruined.
email mjsmith at hgmp.mrc.ac.
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