How to break out bacteria on the plate

Jung Hoon Ahn jhahn at
Tue Jun 11 20:58:46 EST 1996

I'm now finding the method to detect a enzyme activity on agar plate.

It is intracellular enzyme and its activity can show halo on the plate.

If we can break out our bacteria on the agar plate as it is,

we can find colonies that have the enzyme.

Is there any method or reagent to break out bacteria on the plate?

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