Phage Display Library

[Michael Benedik]

In article <Dst1K4.L64 at ncifcrf.gov>
pnh at cutterjohn.ncifcrf.gov (Paul N Hengen) writes:

> I'm beginning a phage display library screening and I have a few
> questions for an expert. I want to pan for a DNA binding protein
> or peptide (fragment of the binding protein) using an immobilized
> DNA fragment.
> 
> 1. Is there an E. coli library available for doing this?
> 

If you are looking at short peptides, then libraries are all synthetic
anyway, so there is no such thing as an E. coli library.

If you are looking at larger peptides (<20aa) then there may be.

> 2. What is the largest size of displayed peptide made?
> 

Large. Entire proteins can be displayed, so certainly 40kDa, and I
think bigger but I can't give you the citation off the top of my head.

> 3. Is there a best vector or kit in your opinion for doing this?
> 

Really depends upon what you are doing. Most vectors are derived from
an original few, so they behave similarly. The choice is a true phage
or a phagemid. Otherwise just a few added bells and whistles for the
most part.


> -Paul.
> 
> --


Michael Benedik
Department of Biochemical Sciences
University of Houston
benedik at uh.edu