ExpressHyb Hybridisation Solution

[Sandra Pena De Ortiz]

On Tue, 11 Jun 1996, Patrick HJ Falckh wrote:

> Lo Netters,
> 
> I got a flyer the other day about a product from Clontech called 
> ExpressHyb, which is a 'new' hybridisation solution which only 
> requires 1 hour for hybridisation runs (Northerns, Southerns etc).
> 
> I would appreciate it if anyone has used the stuff if you could email 
> me your impressions (good or bad) and what pitfalls you may have come 
> across (although companies never tell us there are any!)
> 
> In appreciation,
> 
> 
> -- 
>                                                                     
>    Patrick HJ Falckh PhD                                 #     #     
>    Key Centre for Applied & Nutritional Toxicology      # # # # #    
>    RMIT - City Campus                                    # Q Q #     
>    GPO Box 2467V, Melbourne  Vic  3001                   #  o  #     
>                                                           # ~ #      
>    Telephone  : 03 9660 3136                             ##<->##     
>    Fax No.    : 03 9663 6087                            #       #    
>    Email      : p.falckh at rmit.edu.au                   # #     # #   
>                                                        ##       ##   
>    "No matter how tall your grandfather                  #  \/  #    
>        was, you have to do your own growing"              # /\ #
>                                                          ooO  Ooo
> 
> 


Hi!

We have been using Express Hyb in the lab for almost a year with no 
problems.  we've used in in southerns, northerns, and colony lifts.  It 
works well for ransdom primed and for end-labelled oligonucleotide 
probes.  We havee needed to extend the prehyb step in some cases but the 
1 hour hybridization always works.  

Good Luck

SPO

UTSA