cheap IPTG ?
Mike Rogers
mrogers at mayo.edu
Tue Jun 11 12:41:19 EST 1996
Another option that you can use instead of IPTG is using lactose as the
inducer. Although it is metabolized by some cell types its cost is
still 5-10x lower than those I have seen here. For more details try
Hoffman et al., Lactose Fed-Batch Overexpression of Recombinant
Metalloproteins in Escherichia coli BL21(DE3): Process Control Yielding
High Levels of Metal-Incorporated Soluble Protein. Protein Expression
and Purification 6, 646-654 (1995). Hope it helps.
Mike Rogers
mrogers at mayo.edu
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