CASTING METAPHOR AGAROSE GELS
wolffie at u.washington.edu
Tue Jun 11 12:20:56 EST 1996
In article <31BD9671.4D02 at acs.tamu.edu>, pjb6578 at acs.tamu.edu wrote:
> I have had a difficult time trying to get consistent
> results running microsatellites on 4% Metaphor
> agarose gel. The gels always run crooked. One side
> of my 34 lane, 3mm gel always runs faster than the
> other. Yet there is no pattern to it; sometimes the
> left side runs faster, sometimes the right, and
> sometimes the middle. I thought the problem was due
> to an unlevel casting tray, but I always use a
> leveler to make sure. I then thought maybe my
> agarose was hardening before it spread out so I tried
> submerging the Bio-rad casting tray under warm water
> (about 65degC) the same temp as the agarose being
> poured. I even tried using a 3% gel but got the same
> results. I have never had so much trouble casting a
> gel before.
> Does anyone out know any tricks to casting Metaphor
> gels consistently? Thanks.
Is the apparatus level, or unevenly cooled, when you run it?
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