PCR-What is in my reaction?
Perry J. Bushong
pjb6578 at ACS.TAMU.EDU
Tue Jun 11 11:51:51 EST 1996
I'm just trying to figure out what is in my PCR
reaction. After amplification, I always find a
stringy, snotty-looking mess in the bottom of all of
my tubes. I put some under the light microscope and
at 400X it appeared as a thin clear ribon. It
doesn't seem to interfere with my reaction, but it
tends to clog up my pipette tips when I'm loading my
gels. My PCR protocol includes: BSA, template,
primers, MgCl, water, buffer, dNTPs, Taq, and I add
some Cresol Red dye to for visibility. I do not use
any mineral oil because my tubes have caps. I guess
the ribbon might be a product of the BSA or the
Cresol Red.
Does anyone have any ideas on what this stuff is, or
how to easily get rid of it?
Thanks.
Perry
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