PCR-What is in my reaction?

Perry J. Bushong pjb6578 at ACS.TAMU.EDU
Tue Jun 11 11:51:51 EST 1996

I'm just trying to figure out what is in my PCR 
reaction.  After amplification, I always find a 
stringy, snotty-looking mess in the bottom of all of 
my tubes.  I put some under the light microscope and 
at 400X it appeared as a thin clear ribon.  It 
doesn't seem to interfere with my reaction, but it 
tends to clog up my pipette tips when I'm loading my 
gels.  My PCR protocol includes: BSA, template, 
primers, MgCl, water, buffer, dNTPs, Taq, and I add 
some Cresol Red dye to for visibility.  I do not use 
any mineral oil because my tubes have caps.  I guess 
the ribbon might be a product of the BSA or the 
Cresol Red. 
Does anyone have any ideas on what this stuff is, or 
how to easily get rid of it?      

More information about the Methods mailing list