FISH and Banding... What is a good technique (R or G??) ?
dauw at ruly46.medfac.leidenuniv.nl
Tue Jun 11 08:46:44 EST 1996
There are a lot of methods published. If you prefer the
G-banding than look at the following paper
Smit, V.T.H.B.M., Wessels, J.W., Mollevanger, P., Dauwerse,
J.G., van Vliet, M., Beverstock, G.C., Breuning, M.H.,
Devilee, P., Raap, A.K. and Cornelisse, C.J. Improved
interpretation of complex chromosomal rearrangements by
combined GTG banding and in situ suppression hybridization
using chromosome-specific libraries and cosmid probes. Genes
Chrom Cancer 3:239-248, 1991.
I prefer the R-banding, but it does involve BrdU
incorporation. The advantage is that you see bands and
signals in one image. However it requires pI and DAPI
staining of the best bands, so you can only use one color.
I used the very nice method of Takahashi et al.;Human
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