CASTING METAPHOR AGAROSE GELS

[STEVEN GERHARDT]

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     Perry,
     
     We always recommend when using MetaPhor to let the gel set a 4C for 30 
     minutes prior to running.  Once you pour the gel and it has solidified 
     at room temp, place in the frig for 30 minutes.  If you had not done 
     this before, it may solve your problems.
     
     You can always check our web site for the latest protocols.
     
     Sincerely,
     Steven Gerhardt
     FMC BioProducts
     Technical Service
     1-800-521-0390


______________________________ Reply Separator _________________________________
Subject: CASTING METAPHOR AGAROSE GELS
Author:  pjb6578 at ACS.TAMU.EDU ("Perry J. Bushong") at Internet
Date:    6/11/96 8:55 AM


I have had a difficult time trying to get consistent 
results running microsatellites on 4% Metaphor 
agarose gel.  The gels always run crooked.  One side 
of my 34 lane, 3mm gel always runs faster than the 
other.  Yet there is no pattern to it; sometimes the 
left side runs faster, sometimes the right, and 
sometimes the middle.  I thought the problem was due 
to an unlevel casting tray, but I always use a 
leveler to make sure.  I then thought maybe my 
agarose was hardening before it spread out so I tried 
submerging the Bio-rad casting tray under warm water 
(about 65degC) the same temp as the agarose being 
poured. I even tried using a 3% gel but got the same 
results. I have never had so much trouble casting a 
gel before. 
Does anyone out know any tricks to casting Metaphor 
gels consistently?  Thanks.
Perry
     
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From: pjb6578 at ACS.TAMU.EDU ("Perry J. Bushong")
Subject: CASTING METAPHOR AGAROSE GELS
Date: 11 Jun 1996 08:55:18 -0700
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