NIH 3T3 Cells

Marcel Hoffmann hoffmann at chem.vu.nl
Thu Jun 13 09:15:41 EST 1996


Hello netters,

I'am experiencing some difficulties transfecting NIH 3T3 
cells. Does anyone have a protocol by which one obtains at 
least an transfection efficiency of 1%. We tried the calcium 
phosphate kit of Promega and had an efficiency less then 
0.1%.

thanks in advance,

Dr. Marcel Hoffmann
Dept of pharmacochemistry
Vrije Universiteit, Amsterdam



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