Lambda DNA prep

John Brennand john.brennand at gbapr.zeneca.com
Thu Jun 13 03:58:18 EST 1996


Chi

The MgCl2 is there simply to prevent the phage particles from bursting 
and releasing their DNA.  MgCl2 helps hold the coat proteins intact.

The protocol was plaigarised 15 years ago from an old CSH 
bacteriological methods manual that we have long since lost so I don't 
know the original references to it !

Glad to hear it is working well for you.

John





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