sequencing gel won't stick to Whatman paper

(Dave Johnston) daj at nhm.ac.uk
Thu Jun 13 02:16:20 EST 1996


It might be worth prewetting your Whatman paper and then blotting it dry 
with tissue. We routinely did this (b4 ABI) and it worked well. 4 things 
to note: (1) if the gel is too wet or dry it will not stick (2) this method 
"relaxes" the paper so that it will not expand on contact with the gel, 
thus avoiding wrinkles and streched zones (same principle as prestreching 
wallpaper before hanging it) (3) if you can see a water film on top of the 
paper after putting it on the gel, it is too wet so blot off excess with 
tissue (4) gently peel up from a corner.

DAJ

David A. Johnston
Executive Secretary to the WHO Schistosoma Genome Network,
Researcher, Biomedical Parasitology Division, 
Dept of Zoology,
The Natural History Museum,
Cromwel8l Road,
South Kensington,
London SW7 5BD,
England
tel 0171 9389297 (from outside UK: 44 171 9389297)
fax 0171 9388754 (from outside UK: 44 171 9388754)
email daj at nhm.ac.uk



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