Long biotinylated DNA binding to magnetic particles

Mr. M.J. Lush mlush at hgmp.mrc.ac.uk
Thu Jun 13 05:27:50 EST 1996


In article <199606130902.SAA29399 at rtcmain.rtc.riken.go.jp>,
piero carninci <carninci at rtc.riken.go.jp> wrote:
>Does anyone have any suggestion or good protocol to bind long, biotinylated
>DNA fragments (10 Kbp) to streptavidin-coated magnetic beads? I know
>already that binding to beads of long fragments can be not so much
>effective. I tried recently to bind at 50C for 30' in high salt buffer, but
>the result was not better than standard protocols, due also to the
>aggregation of beads under high salt condition. Any good idea?
>

	I've just started using Dynal streptavidin beads,  in their
Techniques book they suggest using 3M LiCl to improve binding of
large fragments (>1kb),  they also showed data that molar binding 
capacity was reduced with DNA length.

(I've no connection with Dynal etc etc)
-- 
Michael

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
NPC rights activist           |      Nameless Abominations are people too.



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