PCR-What is in my reaction?

Nick Jacobsen jacobsen at cf.ac.uk
Thu Jun 13 04:19:33 EST 1996


Hi there,
        Concerning your PCR reactions- In the past I have used BSA for 
pulsed field digests. Prior to using the BSA I put it in a boil bath for 
10 minutes (to rid it of nuclease activity), this turned the BSA into a 
stringy mess similar to what you described. I consequently had to disgard 
it. Im not sure that you put enough BSA in your PCR reaction to cause a 
visible precipitate but it might be possible. I never use BSA in PCR 
reactions myself.

I hope this helps

Nick Jacobsen. 




More information about the Methods mailing list