Isolation of "hot" oligos

Joachim Kremerskothen kremers at
Thu Jun 13 04:18:20 EST 1996

Hi netters,

we have a problem with the isolation of radioactive-labeled oligos
(20mers) after T4 kinase phosphorylation. Ethanol precipitation and gel
filtration with Pharmacia`s G50 columns didn´t work very well (maybe
because of the lenght of the oligos?). Are there other and better methods
for the isolation?

Dr. Joachim Kremerskothen
Institut for experimental Pathology
University of Muenster
Mendelstrasse 11
D-48149 Muenster
phone ++49 251 838615
fax ++49 251 838616

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