Isolation of "hot" oligos

Joachim Kremerskothen kremers at uni-muenster.de
Thu Jun 13 04:18:20 EST 1996


Hi netters,

we have a problem with the isolation of radioactive-labeled oligos
(20mers) after T4 kinase phosphorylation. Ethanol precipitation and gel
filtration with Pharmacia`s G50 columns didn´t work very well (maybe
because of the lenght of the oligos?). Are there other and better methods
for the isolation?

-- 
Dr. Joachim Kremerskothen
Institut for experimental Pathology
University of Muenster
Mendelstrasse 11
D-48149 Muenster
Germany
phone ++49 251 838615
fax ++49 251 838616



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