NIH 3T3 Cells

Curt Ashendel ashendel at aclcb.purdue.edu
Thu Jun 13 09:35:07 EST 1996


On Thu, 13 Jun 1996 15:15:41 +0100, Marcel Hoffmann   
<hoffmann at chem.vu.nl> wrote:
>I'am experiencing some difficulties transfecting NIH 3T3 
>cells. Does anyone have a protocol by which one obtains at 
>least an transfection efficiency of 1%. We tried the calcium 
>phosphate kit of Promega and had an efficiency less then 
>0.1%.

Use the CaPO4 protocol as written in Current Protocols in Mol. Biol.
If the plasmid is squeaky clean it gives a transfection rate of  8 to 
15% using beta-gal staining. 


Curt Ashendel
Purdue University, West Lafayette, IN
ashendel at aclcb.purdue.edu



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