Isolation of "hot" oligos

vilimf01 at vilimf01 at
Thu Jun 13 18:35:20 EST 1996

Use G25, G50 will eat your 20mers.  I have used G25 for cleaning up my
labeled oligo's with success on many occasions.

					Usual Disclaimers	SVEN

In article <kremers-1306961122030001 at>, kremers at (Joachim Kremerskothen) writes:
> Hi netters,
> we have a problem with the isolation of radioactive-labeled oligos
> (20mers) after T4 kinase phosphorylation. Ethanol precipitation and gel
> filtration with Pharmacia`s G50 columns didn´t work very well (maybe
> because of the lenght of the oligos?). Are there other and better methods
> for the isolation?
> -- 
> Dr. Joachim Kremerskothen
> Institut for experimental Pathology
> University of Muenster
> Mendelstrasse 11
> D-48149 Muenster
> Germany
> phone ++49 251 838615
> fax ++49 251 838616

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