Isolation of "hot" oligos

vilimf01 at mcrcr6.med.nyu.edu vilimf01 at mcrcr6.med.nyu.edu
Thu Jun 13 18:35:20 EST 1996


Use G25, G50 will eat your 20mers.  I have used G25 for cleaning up my
labeled oligo's with success on many occasions.

					Usual Disclaimers	SVEN

In article <kremers-1306961122030001 at ifepmac3.uni-muenster.de>, kremers at uni-muenster.de (Joachim Kremerskothen) writes:
> Hi netters,
> 
> we have a problem with the isolation of radioactive-labeled oligos
> (20mers) after T4 kinase phosphorylation. Ethanol precipitation and gel
> filtration with Pharmacia`s G50 columns didn´t work very well (maybe
> because of the lenght of the oligos?). Are there other and better methods
> for the isolation?
> 
> -- 
> Dr. Joachim Kremerskothen
> Institut for experimental Pathology
> University of Muenster
> Mendelstrasse 11
> D-48149 Muenster
> Germany
> phone ++49 251 838615
> fax ++49 251 838616



More information about the Methods mailing list