DNA extraction from urine

gturbett at eosin.path.uwa.edu.au gturbett at eosin.path.uwa.edu.au
Thu Jun 13 20:00:02 EST 1996


Does anyone have a good method for extracting DNA 
from urine sediment?
The urines collected in our department are collected 
into a bottle containing carbowax (PEG).
Most times, this precipitates down when the sediment is 
collected, and forms a large pellet, irrespective 
of how many cells are actually present.
We want to extract DNA from the sloughed-off cells 
to use in PCR. 
Does anyone have a reliable method for this?
Gavin Turbett.



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