PCR-dilema!

paola velasco al701032 at MAIL.MTY.ITESM.MX
Thu Jun 13 16:25:57 EST 1996


Hi, I need some advice, could you help me?

I have to amplify a fragment of 86 bp. I have two options: 1) Design 
two primers of about 20-24 bases and run a PCR. or 2) Design two 
oligonucleotides of 48 bp that overlap in 9 bases and do an extension 
with a polymerase. Do you have any advices or experiences about this?

	Thanks,    Paola



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