DIG vs. biotin: Preferencess?
John J Weiland
jweiland at badlands.NoDak.edu
Thu Jun 13 14:45:45 EST 1996
I would appreciate a quick word from anyone who has compared the DIG vs.
biotin labelling/detection systems for chemiluminescent Northern/Southern
blotting. Although I am not using proprietary (commercial) blocking
preparations, the blockers that I am using are not adequate for background
elimination (Hybond N+ membrane, 5% SDS in prehyb/hyb, biotinylated
riboprobe, 5% SDS in streptavidin-alk-phos binding). Any comments will be
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