Freeze and Squeeze
horton at biosci.cbs.umn.edu
Thu Jun 13 11:51:08 EST 1996
Kevin Simcox (simcox.3 at osu.edu) wrote:
: > Ann M. Yezerski wrote:
: > >
: > > Does anyone have the standard protocol for "Freeze and Squeeze" to remove
: > > DNA from an agarose gel? If so, e-mail me at ayezersk at moose.uvm.edu.
: Take a 500 ul microfuge tube and make a small puncture in the bottom
: using a small gauge syringe needle. Place a small amount of
: silconized glass wool in the bottom. Cut out the band of interest and
: place into the 500 ul microfuge tube. Then place the 500 ul microfuge
: tube into a 1.5 ml microfuge tube. Freeze at -70 C for 20 min. Spin for
: 10 min and collect liquid containing DNA fragment. The DNA fragment can
: be equilibrated in a 0.3 M NaOAc, 0.01 M MgCl2 solution prior to loading
: the microfuge tube. Just add 2.5 vol of ethanol after centrifugation to
: ppt the DNA fragment.
Tautz, D. and Renz, M. (1983) Analytical Biochemistry 132:14.
Robert M. Horton
http://188.8.131.52 Have a :) day
"Scotty, try flushing the radioactive waste into the ventilation system!"
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