sequencing gel won't stick to Whatman paper
hroychow at NMSU.EDU
Thu Jun 13 10:25:47 EST 1996
On 12 Jun 1996 jlight at SICKKIDS.ON.CA wrote:
> After I run a sequencing gel (using 35S-dATP for labelling) I have a lot of
> problems getting the gel to stick to Whatman paper. I don't fix the gel
> after the run, because I've found that it's not necessary to get good
> autorads. Unfortunately, and perhaps because I skip the fixing step, my
> gels don't always stick when I overlay them with Whatman paper (sometimes
> they do, sometimes they don't). I've tried spraying the gel with water and
> blotting off the excess with Kim wipes prior overlaying with Whatman, which
> helps sometimes, but it's no guarantee. Sometimes part of the gel sticks
> and part doesn't, so the gel ends up ripping. Advice from anyone who
> routinely transfers their gels without fixing would be greatly appreciated.
It's all in the wrist =:)
Hiranya S. Roychowdhury
Plant Genetic Engineering Lab.
Box 3GL, NM State Univ.
Las Cruces, NM 88003
Phone: (505) 646-5785
hroychow at nmsu.edu
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