Glutathion in Bacterial Media

[Achim Recktenwald]

corboy at utdallas.edu wrote:
> 
> Seeking knowledge of the effects of glutathion in bacterial media.  The
> purpose is analagous to adding 2-ME or DTT to solutions when purifying a
> protein by standard methods.  My protein is secreted into the medium, and
> I am trying to maximize the availability of a single free Cysteine SH
> group by protecting it (anti-oxidant).  To my knowledge, you cannot use
> 2-ME or DTT in culture because they are membrane permeable and toxic.  As
> far as I know, glutathion is not membrane permeable, but can be imported
> by bacteria.  What I want to know is how much glutathion can I add to
> bacteria growing in liquid culture to provide SH protection, but not
> affect growth or reduce existing disulfide bonds, like you would use 2-ME
> for in purification. Thanks in advance for any help I may get.
> 
> Mike Corboy
> University of Texas at Dallas


Ever thought of adding cysteine to the medium?

Achim