Fast purification of protein from DNA

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Fri Jun 14 10:50:18 EST 1996


nobody at nowhere wrote:
> 
> I have a problem of purification an enzyme from DNA inpurities. Do you know
> a fust and reliable method to solve the problem for less then 1ml volume of an
> enzyme solution without loss of protein's bioactivity?
> Thanks in advance,
> Dima Mozzherin

Maybe add a little protamine sulfate, it binds to and precipitates DNA, 
but it will also precipitate some proteins, I use it to pull out PDH. I 
don't have any idea what conditions to use for getting rid of DNA 
though.
-- 
>>>>>>--------------------------->
>Ron Tate                                                                   
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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