Acrylamide Gel setting too quickly -- IDEAS??
jkropp at dnai.com
Fri Jun 14 20:31:51 EST 1996
About 15yr ago some smartass from down the hall wandered in while I
was cursing the water aspirator and asked "why are you degassing?
That's what God made APS for."
I use 60ul 25% APS for 30ml gel, and haven't degassed since. And I
store the APS at -20 in 1ml aliquots. After ~10 freeze-thaw cycles I
have to go to a fresh tube of the stuff. It keeps at least a yr at
Hope this helps, Jerry
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