Invitrogen's pZero plasmids
tmagee at earthlink.net
Sat Jun 15 17:20:39 EST 1996
In article <friedmrd-0806961402290001 at netnews.upenn.edu>,
friedmrd at dunx1.ocs.drexel.edu (Richard Friedman) wrote:
> Has anyone tried using the pZero plasmid for cloning. Does the killing
> function work well?
I tried the Kan version and it worked but not as well as they advertise.
I typically screen 12 minipreps and get 2 or 3 good clones. It appears
that the cut vector must be used immediately. Within a few days the
vector gives too many false positives to be useful.
More information about the Methods