Invitrogen's pZero plasmids

[Tom Magee]

In article <friedmrd-0806961402290001 at netnews.upenn.edu>,
friedmrd at dunx1.ocs.drexel.edu (Richard Friedman) wrote:

> Has anyone tried using the pZero plasmid for cloning. Does the killing
> function work well?


I tried the Kan version and it worked but not as well as they advertise. 
I typically screen 12 minipreps and get 2 or 3 good clones.  It appears
that the cut vector must be used immediately.  Within  a few days the
vector gives too many false positives to be useful.