Invitrogen's pZero plasmids

Tom Magee tmagee at
Sat Jun 15 17:20:39 EST 1996

In article <friedmrd-0806961402290001 at>,
friedmrd at (Richard Friedman) wrote:

> Has anyone tried using the pZero plasmid for cloning. Does the killing
> function work well?

I tried the Kan version and it worked but not as well as they advertise. 
I typically screen 12 minipreps and get 2 or 3 good clones.  It appears
that the cut vector must be used immediately.  Within  a few days the
vector gives too many false positives to be useful.

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